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Additionally, recent clinical evidence has demonstrated that newly diagnosed hypothyroid individuals exhibit elevated malondialdehyde (MDA) and decreased total antioxidant capacity, supporting the link between impaired antioxidant status and heightened oxidative stress (Sultana et al

Western blot analysis and Coomassie Brilliant Blue (CBB) staining Samples were boiled in sample loading buffer at 100 C for 10 min, separated by SDS-PAGE, and transferred onto NC membranes for blotting

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