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doi:10.1001/archinte.156.9.925
Investigating Liver Detoxification and Methylation A targeted history and laboratory assessment may help distinguish excessive exposure from impaired methylation, elevated SAH, poor transsulfuration, low glutathione, biliary dysfunction or gut-derived toxic burden
Like you, I have, prior to B12 deficiency, been fit and active
the lysine residue preceding the active site, which forms electrostatic interactions with the carboxyl group of the glycine in GSH, the active site cysteines and polar amino acids interacting with the carboxyl group of the glutamate in GSH are conserved in most ROXYs, suggesting that GSH binding might be required for activity
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