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Thangavelu T, Lyden E, Shivaswamy V
The ALDH1L1 encoded enzyme was originally identified as 10-formyl tetrahydrofolate dehydrogenase (FTHFD
The role of bile Acid excretion in atherosclerotic coronary artery disease
These enzymes can function in either direction, NADPH oxidation, or NADP + reduction dependent upon the redox state
Western blot analysis involved preparing whole cell lysates using RIPA lysis buffer, quantifying protein concentration via BCA assay, followed by SDS-PAGE, transfer to nitrocellulose membranes, incubation with primary and HRP-conjugated secondary antibodies, and detection using an ECL solution [1]
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