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Permanent hair reduction is defined as the long-term, stable reduction in the number of hairs re-growing when measured at 6, 9, and 12 months after the completion of a treatment regime, as well as treatment of benign pigmented lesions (sun spots and brown spots), wrinkles, and vascular lesions (such as port-wine stains, hemangiomas, telangiectasias)

Antibiotic medium (MEM, 2% PS, 12.5 g/mL amphotericin B, 0.5 mg/mL gentamicin sulfate) was added and the cells were cultured in an incubator (28C + 5% CO2) for 2 hours, with a medium change every 30 minutes

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