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doi: 10.3389/fimmu.2025.1610368 Received 11 April 2025 Accepted 26 June 2025 Published 15 July 2025 Volume 16 - 2025 Edited by Li-Tung Huang, Kaohsiung Chang Gung Memorial Hospital, Taiwan Reviewed by Beata Kasztelan-Szczerbinska, Medical University of Lublin, Poland Edit Posta, University of Debrecen, Hungary Updates Copyright 2025 Migliorisi, Gabbiadini, Dal Buono, Ferraris, Privitera, Petronio, Bertoli, Bezzio and Armuzzi
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Once reconstituted, store the vial under refrigeration at 4 C (39 F)
For drug treatments, compounds were added 2-3 hours post-infection, except for invasion assays, in which case cells were pre-treated with the drug 16-24 h before infection
These neurons integrate energy balance signals and coordinate feeding behavior
Preparation of test compounds for in vitro experiment Adding test compounds to solutions for either static or perifusion protocols involved making up a stock solution and then adding a small volume to the wells (for static) or inflow buffer (for perifusion)
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