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Centrifugation of Fresh leaf sample grounded in potassium-phosphate extraction buffer (0.1 M, pH 7, 5cm 3 ), Triton X 100 (1%), EDTA (3 mM), PVP (1%) was done at 4 C for 10 min at 7800 g
doi: 10.3390/ani11061627 12 BarcelosS
Methods In order to improve the treatment efficiency of HCC, based on in situ growth of a copper metal organic framework on mesoporous organic silica nanoparticles, dual pH- and glutathione (GSH)-responsive multifunctional nanocomposites were synthesized as nanocarriers for enhanced HCC therapy
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