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The first evidence that eNOS uncoupling may be a pathophysiologically relevant phenomenon was provided by in vitro studies in which it was demonstrated that native low-density lipoprotein 81 and even more pronounced oxidized low-density lipoprotein 82 are able to stimulate endothelial superoxide production and that this phenomenon is inhibited by the NOS inhibitor N G -nitro-L-arginine methyl ester, pointing to a causal role for eNOS in superoxide production

First, we clean the vial tops with alcohol swabs