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After the incubation period (1 h), the solution was centrifuged for five minutes (10,000 rpm) at room temperature

Fabrication of Lepidium sativum extract loaded nanospanlastics The development of LS nanospanlastics was carried out by using the ethanol injection approach based on the previous method described by Kakkar and Kaur 29

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L-ascorbic acid (the most potent form of topical vitamin C) requires a low pH, typically around 2.5 to 3.5, to remain stable and penetrate the skin