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For lipid peroxidation level, Cells were treated with the fluorescent dye BODIPY 581/591 C11 (D3861, Thermofisher Scientific), which inserts into lipid membranes and allows for quantitative assessment of oxidized versus non-oxidized lipids by fluorescing green or red, respectively

Fatty Acid Amide Hydrolase Blockade Attenuates the Development of Collagen-Induced Arthritis and Related Thermal Hyperalgesia in Mice

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Thiamine status should be checked at baseline and every 3 months in long-term users ALA can deplete thiamine and trigger neuropathy paradoxically resembling the deficit it was given to correct