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Briefly, 2 10 5 cells were cultured in ultra-low attachment six-well plates using serum-free DMEM/F12 medium supplemented with 1% N2, 2% B27, 15% glucose, 1 g/mL hydrocortisone, 5 g/mL insulin, 10 ng/mL epidermal growth factor (EGF), 10 ng/mL basic fibroblast growth factor (bFGF), and 0.2% heparin (Table S5)

Widely used as an active ingredient in cosmetic and skincare formulations

doi: 10.1038/ijo.2008.202 46 ManciniADi SegniC

The evidence for pre-exercise collagen synthesis stimulation makes me more interested in timing it before workouts than in using it as a stand-alone daily tonic