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Ibuprofen (1.5 M) or 3-PLG-ibu and 3-PLG-ibu-A-GSH nanocarriers (100 g/ml) were added as co-treatments

To make the hEC surface charge more positive the glycocalyx of hECs was digested with neuraminidase enzyme (1 U/ml, 1-h pretreatment) or cultures were treated with cationic lipid 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH

Spermidine: clinical studies Spermidine has also been studied extensively in human populations, with a focus on its ability to improve cognitive function, promote longevity and reduce the risk of age-related diseases (Table 4)

Common dosing: 0.11 mg/kg total peptide via subcutaneous injection, split into 10100 g/kg per component

Sterile water lacks preservatives, meaning each needle puncture increases contamination risk