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Membranes were incubated overnight at 4 C with Cleaved Caspase-3 (Asp175) (Cell Signalling Technology) and with peroxidase-linked anti-rabbit IgG or anti-mouse IgG secondary antibodies (GE Healthcare Life science) for 1 h at room temperature

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GLP-1 RAs now present a promising therapeutic approach for AS in a similar manner to other relatively recently discovered nephroprotective drugs, such as SGLT2 inhibitors and finerenone [63,64,65]