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The method uses a gradient of solvents with an initial ratio of 10:90 (v/v, aqueous: organic phase) at a flow rate of 1 mL/min linearly changing to 90:10 over 10 min and kept constant for one minute

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3 for a plate diagram of the solution arrangement

However, patients should diligently check their insurance plans drug formulary to confirm whether Tirzepatide is included and understand the extent of coverage, which could impact the drugs availability to them