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The pivotal moment arrived in 198687, when Joel Habener and Svetlana Mojsov at Massachusetts General Hospital identified GLP-1, a truncated fragment of a glucagon-related protein with powerful insulin-stimulating properties

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The columns were washed with 2 1 ml BSA solution, which was also collected, and then, the suspension was centrifuged at 300 g for 5 min at 4C