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Mtodos modernos de cromatografia lquida acoplada espectrometria de massa (LC-MS/MS) permitem a identificao de pequenas concentraes de peptdeos e seus catablitos na urina e no sangue
GPX was measured adding cell extract to 50 mmol/l potassium phosphate pH 7.0 containing 1 mM EDTA, 1.5 mM sodium azide, 0.15 IU of purified glutathione reductase from bakers' yeast, 0.45 mM GSH, 0.2 mM NADPH, 1.2 mM cumene hydroperoxide One unit of GPX was defined as the amount of enzyme which produces 1 mol of GSSG (oxidized glutathione)/min at 25C
Peptide amount: 10,000 mcg total in the vial
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Involvement of glutathione peroxidases in the occurrence and development of breast cancers
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