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Levels in the 400-500 range usually indicate a deficiency, especially if coupled with the symptoms above
While animal studies consistently demonstrate beneficial effects across diverse injury models and tissue types, the absence of adequately powered, controlled human clinical trials prevents definitive conclusions about efficacy in human disease
doi:10.1038/s41598-021-92753-4
For most people the honest answer is: often at first, then much less often once your stores are refilled
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