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For tumour xenograft models, 1 10 6 HCC-1806 cells or 5 10 6 PC3 cells were injected into the right fourth mammary fat pad or hind flank of athymic nude NU/J mice (The Jackson Laboratory, 002019) in 100 l sterile PBS or a 1:1 mixture of cell suspension in PBS and Matrigel (Corning, 356237) using a 29-gauge 1 ml insulin syringe

Reconstituting 5 mg in 2 mL yields a 2.5 mg/mL (2500 ug/mL) stock, and changing the diluent volume scales the concentration proportionally

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Treating your reconstituted solution with the same care as your initial powder is the final step in ensuring reliable, repeatable results

But getting enough cysteine from food alone can be tricky, which is why many people turn to NAC supplements