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213,216 NAD + -dependent modification of metabolic enzymes Beyond serving as a hydride-donating coenzyme for metabolism, NADH/NAD + also acts as co-substrate for the sirtuins-mediated post-translational modification of metabolic enzymes including acetylation, ADP-ribosylation, succinylation and malonylation

Competing interests The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest

This method is valued for its specificity, effectiveness, environmental friendliness, and safety

Unnecessary parental anxiety is one of the controversies regarding anesthesia-related neurotoxicity (Barnes, 2020)

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