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Izon Science, Medford, MA, USA) and ultrafiltration (Amicon Ultra-2 Centrifugal Filter Unit30 kDa cutoff

If no validated condition is available, temporary storage of unopened lyophilized material at approximately 20C in a tightly sealed, dry, light-protected container may be used as a conservative laboratory holding condition, but it must not be presented as a validated shelf-life claim

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The N I C system (News- paper in the Classroom) is organized by A N P A (American Newspaper Publishers Association)