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Sections were washed in PBS-0.05% Tween-20 and incubated with secondary antibodies (Alexa 594 anti-Rabbit and Alexa 488 anti-Chicken, Thermo Fisher Scientific) for 1 h in room temperature
Building to therapeutic concentration levels takes weeks, and expecting results in days leads to premature conclusions about product quality
Which labs or vitals should I monitor
Individuals with restrictive eating disorders, fasting behaviors, or weight-driven purging may be at increased risk of symptom reactivation and are not appropriate candidates
Shestakova, E
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