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This is the theoretical basis for endogenously supplied gamma-glutamylcysteines ability to raise GSH levels, as being the product of the GCL enzyme, it can be taken up by cells intact, where it feeds directly into glutathione synthase to be converted to glutathione, effectively bypassing the dysfunctional GCL
The specific primers for RRM2 amplification were used as follows: Sense, 5-TTGCCTGTGAAGCTCATTGG-3 and antisense, 5-CCTCTGATACTCGCCTACTCTC-3
They do not address underlying metabolic dysfunction and stop working when discontinued
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