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Throughout these evaluations, GHK appeared to lower ROS levels induced by tert-butyl hydroperoxide (t-BOPD), a chemical known to promote oxidative stress within cells
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42 of those peptides were located in their protein precursors conserved structural domains and they also had secondary structures
Materials H-His(Trt)-OMeHCl, H-Lys(Boc)-OMeHCl, H-Lys-OMe2HCl, ((1H-Benzo[d][1,2,3]triazol-1-yl)oxy)tri(pyrrolidine-1-yl)phosphonium hexafluorophosphate(V) (PYBOP), 2-(1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HBTU), 1-hydroxy benzotriazole hydrate (HOBT), GHK and 4-carboxyphenyl boric acid were obtained from Bide Pharmatech Ltd (Shanghai, China)
