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Then push the plunger forward to exactly the right mark, expelling any air bubble that entered the syringe

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From experimental data of kinetic measurements of BRET, following vehicle and baseline corrections, quantification of induced responses from individual peptide concentrations was determined by calculating the net area under curve from the 10 min response (AUC 0-10min) ) in GraphPad Prism version 9.0.1 (GraphPad Software Inc., San Diego, CA)

Regulatory oversight is minimal in most jurisdictions, and compounded peptide sources lack standardized quality assurance comparable to FDA-regulated pharmaceuticals