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In this assay, the 30 min incubation duration was chosen to be optimal because of the inherently cytotoxic nature of CuCl 2 and Cu(OAc) 2 towards HaCaT keratinocytes, as they were observed to cause substantial cell death at a duration longer than 30 mins
It does increase infection risk and delay healing, which is why leaving blisters intact is recommended, but it does not cause the reaction to spread
If confirmation of catheter placement is desired, contrast media can be injected through the catheter and radiographic images can be studied
-Lactam/-lactamase inhibitor combinations: an update
Practical take For clinicians, this reinforces a more nuanced approach: Match the drug to the patient profile Look beyond average weight loss alone Consider tolerability, adherence, comorbidities, access, and long-term strategy The obesity pipeline is getting deeperand that is good news for patients
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