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A typical sample preparation protocol for clinical measurement of IGF-1 using LC-MS/MS may involve several steps, such as acid dissociation of the IGF-1/IGFBP complex, the use of IGF-2 to prevent re-association, liquid extraction using organic solvent, disulfate-bound reduction, cysteine alkylation, and overnight trypsin digestion of IGF-1 [Citation91]
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Others find that a specific day of the week, aligned with their lowest-stress period, produces the best tolerance
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