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However, our study focuses specifically on pharmacological aspects of GLP-1R agonism, with prolonged incubations deliberately chosen to mimic in vivo drug exposure, and knockdown of both -arrestin isoforms to better approximate the response to exendin-phe1 and related agonists

Both had simultaneous publications in New England Journal of Medicine and Nature, respectively

Wide-targeted metabolome analysis identifies potential biomarkers for prognosis prediction of epithelial ovarian cancer

Briefly, sodium bicarbonate, glucose, sodium pyruvate, and all 20 amino acids (except cystine and serine), biotin (B4639, Sigma), and vitamin B12 (V6629, Sigma) were added to the DMEM medium (#D9800-28, USBiological, Salem, MA) to match standard IMDM, supplement with 15% human AB serum and 15% dialyzed FBS (#SH300790340, Cytiva Hyclone), along with 10 ng/mL interleukin-3, 50 ng/mL stem cell factor and 2IU/mL erythropoietin

Sci Rep 14 , 24433 (2024)