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The mechanism behind these effects is often attributed to the direct or indirect antioxidant activity of NAC and redox regulation of signal transduction, including WNT/-catenin, TP53, and PI3K/AKT pathways
Cultures were pulse-labeled with 10 M BrdU (a nucleoside analog that competes with thymidine for incorporation into DNA) in DMEM-10% HS for the final 2-h period of 48-h in culture, followed by immunocytochemistry for detection of BrdU using G3G4 anti-BrdU mAb (1:100 dilution) and HRP-conjugated anti-mouse IgG Ab (1:500 dilution)
Kessler MJ et al
Conventional oral glutathione can be degraded by gastric acid and digestive enzymes before it reaches systemic circulation, which historically raised concerns about its effectiveness
Costa C, Incio J, Soares R
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