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Nasal Spray Delivery: Convenient and effective, ensuring quick absorption and systemic action

To construct vectors for the Y2H assays, LET7 , LET7 TPR , LET7 PPase , HOP1 , HOP1 TPR1 (600 bp, from 1 to 600), HOP1 TPR1 / 2a (1,140 bp, from 1 to 1140), HOP1 TPR2a / 2b (1,116 bp, from 601 to 1716), HOP1 TPR2b (576 bp, from 1141 to 1716) or CRCK3 SP (1,440 bp, from 91 to 1530, without the signal peptide) were amplified via PCR from the pHBT -p35S :: LET7-HA , pHBT -p35S :: HOP1-HA or pHBT -p35S :: CRCK3-HA vectors and subcloned into the pGADT7 and pGBKT7 (Clontech) vectors

Liu L, Zhao Z, Lu L, Liu J, Sun J, et al

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Obstet Gynaecol Reprod Med 28:714