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Serum limitation-induced organ culture model of keloids and apoptotic induction of FOXO4-DRI in situ The keloid tissues were cut into 4-mm diameter cylinders using a corneal trephine for organ culture and cultured in a DMEM containing 2% FBS for 10 days to construct the serum limitation-induced senescent organ culture model of keloid tissues

To investigate the role of GSS in male reproduction, we constructed Gss Flox/Flox mice by inserting two LoxP site to the left and right of exon 3 (Fig