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doi:10.1371/journal.pone.0119479 41

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indicated that GPX4 and GSH levels were decreased, and cellular iron and MDA levels were increased in a mouse model of myocardial ischemiareperfusion (MIR, ischemia 30 min and reperfusion 24 h) injury [90]

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All of these ingredients are considered nervine botanicals that have been traditionally used as a sleep aid due to their relaxant properties and ability to help promote quality sleep, without causing morning drowsiness.28 The sedative and sleeppromoting properties of valerian, passion flower, and lemon balm may be influenced by the interaction of the volatile oils and bioactive constituents with GABA receptors, modulating GABAergic signaling.29 Although research on valerian root is inconsistent and more clinical studies are needed, a systematic review and metaanalysis of 60 studies suggest that it may be an effective herb to help promote sleep.30 In vitro and clinical studies showed that valerian root may help reduce sleep latency and improved subjective sleep measures.29 In vitro and animal models found that passion flower reduced sleep latency and increased sleep duration, and lemon balm improved overall sleep quality by decreasing GABA transaminase activity, which metabolizes GABA.29 In a clinical trial (n = 60) of elderly people, the group receiving 200 mg of chamomile extract twice daily for 28 days showed significantly improved sleep quality compared to the control group.31 * These statements have not been evaluated by the Food and Drug Administration