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10.1016/j.sleep.2020.10.018 34 YamatsuA.YamashitaY.MaruI.YangJ.TatsuzakIJ.KimM
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D., and Jun, J
The Boc-protected cystine-1,1- 13 C was reacted with two equivalents of glycine tert -butyl ester (OtBu) hydrochloride using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide monohydrochloride and 1-hydroxybenzotriazole to produce the N and C-protected dipeptidyl (Boc-Cys-Gly-OtBu) disulfide derivative
Risks of Improper Handling Loss of activity Aggregation and insolubility Bacterial contamination Chemical degradation Inconsistent results Pre-Reconstitution Preparation Sterility: Always work in a clean environment
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