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The disadvantage is the need for injection, which some people find uncomfortable or inconvenient

33 At a certain temperature and pressure, carbon dioxide is continuously injected into the reactor to make the fluid reach the supercritical stage above the critical temperature

While the primary focus remains on CF-relevant systems, techniques for measuring intracellular ROS have also been applied in studies investigating the effects of compounds on oxidative status in other cell types, such as platelets, in inflammatory contexts, highlighting the broader applicability of these methodologies in understanding redox biology (Gziut et al., 2013)

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