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After centrifugation at 500g for 15 min (70), semen samples from each group ( n = 3) were collected with the number of frozen-thawed spermatozoa adjusted to a final concentration of 1 10 6 sperms/ml, added to the above fertilization droplet containing MII oocytes (repeated in triplicate, 205 oocytes for the NC group, 210 oocytes for the GSH group, 220 oocytes for the SeNPs group and 217 oocytes for the GSH + SeNPs group, repeated in triplicate) and cultured in a CO 2 incubator (38.5C, 5%CO 2 and 5%O 2 ) for 18 h
Statistical analysis All statistical analyses were conducted by utilizing GraphPad Prism 10 (GraphPad Software)
Glutathione oral supplements come in capsules or tablets that dissolve under the tongue
10.1002/ana.20484 380 Farrer M., Stone J., Mata I
Recruitment of CCR6-expressing Th17 cells by CCL 20 secreted from IL-1 beta-, TNF-alpha-, and IL-17A-stimulated endometriotic stromal cells
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