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To calibrate the measurement baseline, a lipid film composed of 20:1PC, 2.5 mol% Ni-NTA, 0.15mol% DOPE-LissRhod B, and 1 mol% TopFluor-Cholesterol was rehydrated with MQ water, sonicated at low power (20% of max), added to MOPS buffer (final concentration, 50mM MOPS pH 7.4, 100mM NaCl), and filtered through a 200nm PTFE filter (Millipore)

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[Crossref] Mossmann M, Wainstein MV, Gonalves SC, Wainstein RV, Gravina GL, et al

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