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Reconstitute Gently: Draw your desired amount of BAC water into the syringe

After different groups of HUVECs are treated accordingly, they are seeded into 24-well plates pre-coated with 150 L/well growth factor-reduced Matrigel (Corning, catalog number 354234) and incubated at 37 C in a cell culture incubator for 12 h

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The collected samples were immediately centrifuged at 3,000 x g at 4C for 10 min to separate the serum