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Lack of activity of recombinant HIF prolyl hydroxylases (PHDs) on reported non-HIF substrates

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Mitochondrial mass assessed by NAO staining in ( a ) HCT116 cells treated with TS-targeting therapies, ( b ) non-small cell lung cancer (NSCLC) cell lines treated with pemetrexed, ( c ) HCT116 cells treated with 5FU, FOLFOX (5FU+oxaliplatin), FOLFIRI (5FU + SN38), or single agent oxaliplatin and SN38, or ( d ) HCT116 cells treated 5FU Torin or rapamycin for 72 h