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Labelled cells were then detached by trypsinization, pelleted, washed once with PBS, resuspended in 300 l ice-cold PBS and fixed by adding 700 l ice-cold absolute ethanol while gently vortexing
the plasma was stored at 80 C until assays were performed
The dual 10mg configuration (20mg total) is exceptionally suitable for extended research timelines and highly controlled experimental designs
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