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In the liver model, proliferating HepaRG cells were used as an adequate alternative to human primary hepatocytes, the "gold standard" for in vitro drug testing, due to their availability, comparable CYP enzyme expression and presence of relevant efflux and uptake transporters 65,66,67

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Although thiamine deficiency is rare in developed countries, it can lead to severe neurological disorders, emphasizing the importance of adequate thiamine intake for optimal health and as treatment when administered promptly (42)

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