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The cells were subsequently treated with DCFH-DA (10 M, Bio-lab) for 1 h in the dark, and washed three times with PBS

4.2 GSH/GSSG Imbalance in Aging and Neurodegenerative Disease Because the brain has high energy demand, abundant oxidizable lipids, and strong dependence on mitochondrial function, glutathione metabolism is frequently studied in aging and neurodegeneration

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SpaMTPs colocalization analysis found m / z 743.546 as the top mass value with spatial location correlating to cluster 5 (Fig