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Py230 cells (provided by Nicholas Webster and Lesley Ellies, University of California San Diego) were maintained in F12K Nutrient Mixture (Corning 10-025-CV) supplemented with 5% Fetal Clone II (Cytiva SH30066.03), 50 g/mL gentamycin (Sigma-Aldrich G1397), 2.5 g/mL amphotericin B (Sigma-Aldrich A2942), and 1 L/mL MITO+ Serum Extender (Corning 355006) at 37 C and 5% CO 2
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With some insurance coverage and a discount coupon from the pharmaceutical manufacturer, Venezia paid about $350 for a months supply of injections after meeting his deductible
Tirzepatide (Zepbound's active drug) is a dual agonist targeting GLP-1 and GIP
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