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In this final step, glutamine synthetase first reacts with gamma-glutamyl-cysteine to form an electrophilic acylphosphate intermediate by transfer of the gamma-phosphate of ATP to gamma-glutamylcysteine, and then the amino group of glycine acts as a nucleophile to attack the acylphosphate intermediate to form glutathione (28
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in diabetic rodents, Fan and colleagues [77] demonstrated that intravitreal exedin-4 injection reduced retinal cell loss, maintained Bcl-2/Bax ratios, whose imbalance is associated with retinal cell apoptosis, prevented activation of pro-apoptotic AKT, and prevented excess reactive gliosis
In brief, cerebral tissue was dissected and chopped into 0.5-1.0 mm fragments, then digested in 0.125% trypsin-EDTA (Gibco, USA) at 37 C for 20 minutes, with gentle agitation every 5 minutes
It is not approved by the FDA for human or veterinary use, and it is not intended to diagnose, treat, cure, or prevent any disease
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