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Briefly, animals were assigned to two groups ( n = 5 male mice per group), vehicle (5% DMSO and 0.5% Kollidon-30 in 100 mM sodium phosphate buffer) or TLC-2716 (1 mg per kg (body weight) daily by oral gavage) for 8 days, after which animals were humanely killed by cardiac puncture, and plasma and tissues were collected for lipid and gene expression endpoints as described below

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