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In the TMRM experiments, NAD(P)H autofluorescence was monitored in 2 mmol/l and 20 mmol/l glucose conditions and later normalised to CCCP levels
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If you are using a liquid liposomal format, you can take it directly or mix it into a small amount of water or juice
That 8-to-72-hour Cmax window is precisely why the 72-hour minimum exists
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