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parkeri division and survival while the other cysteine-containing molecules did not is unclear but could likely be due to import rates, as NAC is better taken up by cells, less prone to oxidation, and for these reasons is widely used both in tissue culture and in people as an antioxidant 47,48

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In addition, GPX4 inhibitors remain challenging to use in vivo owing to their poor bioavailability, and pharmacological inhibition of GPX4 carries a substantial risk of systemic toxicity, as demonstrated in GPX4 knockout mouse models 25,26,27 Thus, modulating the CoQ 10 redox cycle, either by inhibiting ubiquinol (CoQ 10 H 2 , reduced) formation or altering oxidoreductase activity, has emerged as an alternative therapeutic strategy for ferroptosis-based cancer therapy