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Different membranes for each primary antibody were used, each then incubated with a pan-H3 loading control antibody and secondary antibodies with intervening three 5-min washes with phosphate buffer saline with 0.1% Tween-20 (0.1% PBS-T)

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258 In a meta-analysis of randomized controlled trials investigating various interventions in women with poor ovarian response undergoing IVF, the pooled findings from two trials using DHEA therapy, at 25 mg three times daily, showed it increased the odds of pregnancy nearly 2.5-fold

Side effects were mostly mild

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