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Nat Rev Mol Cell Biol 19 , 654672 (2018)
Splitting the dose or adding a second daily injection is not part of the clinical protocol and may not improve outcomes
Following 30 min of incubation at 4 C, extracts were cleared by centrifugation (17,000 g , 20 min, 4 C) and then supernatants were analysed by liquid chromatography and mass spectrometry (LCMS)
Needles and syringes for injection ordered separately.
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