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In one mouse study, researchers found that the peptide reduced the pain felt by the animals in their paws when placed on a mildly hot plate. The authors explained that this could be because GHK is similar in structure to cimetidine, a histamine H2 receptor antagonist sometimes given to reduce pain in certain digestive conditions.3 10 In rodents, doses as low as 0.5mg/kg were found to be effective in delivering these anti-pain effects.25 In a 70kg human, an equivalent dose would be around 5.6mg, though thats likely higher than necessary: pharmaceutical-grade GHK-Cu normally ranges from 0.5 mg to 2.5mg per injection

Between 2 and 1 h before the dark phase, rats were injected with either MTII or vehicle in randomized order using the same procedure as described previously ( POMC and AgRP in Situ Hybridization In a separate study, using the same dietary protocol, rats were subjected again to CHOW ( n = 7), fcHS ( n = 7), fcHF ( n = 7), or fcHFHS ( n = 7) for 4 weeks

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