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6.4 Immunomodulatory therapies Immunomodulation has emerged as a promising investigational approach in PD, supported by evidence that microglial activation, inflammasome signaling, and peripheral immune dysregulation may contribute to neurodegenerative processes

doi: 10.1007/s11033-023-08403-7 56 GhioAJCarterJDRichardsJHRicherLDGrissomCKElstadMR

Plasmid for single-stranded adeno-associated viral vectors 2/8 The plasmid pAAV-MCS-hAAT/EGFP was designed for maximum transgene expression and includes the hepatic locus control region from the ApoE gene (ApoE-HCR), a liver-specific 1-antitrypsin promoter (hAAT promoter), coagulation factor IX intron A (FIX intron A), a 3' mRNA transcription termination/polyadenylation signal (bghpA), and a reporter gene encoding enhanced green fluorescent protein (EGFP), as previously described (Miao et al

Letting lyophilized powder warm to room temperature before adding water doesn't improve dissolution and adds unnecessary time at non-refrigerated conditions