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Within controlled laboratory settings, 5-Amino-1MQ is utilized to study how NNMT inhibition alters intracellular nicotinamide handling and affects downstream metabolic signaling pathways, including those related to NAD+ homeostasis, mitochondrial dynamics, and adipocyte functionality

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A study conducted at Monash University found that AOD-9604 reduced body fat in obese animals by 50% over a 19-day treatment period without changes in food intake or lean muscle mass

After initial repletion, a maintenance dose is given, often 1,000 mcg intramuscularly every month or every 2 to 3 months, to prevent recurrence of deficiency

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