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GST tagged fusion proteins bind to glutathione agarose (column 2) and, after washing, are eluted with elution buffer with excess glutathione (column 3)

Carcinogenesis 28:193745, PMID: 17698970 Neuschwander-Tetri BA, Bellezzo JM, Britton RS, Bacon BR, Fox ES (1996) Thiol regulation of endotoxin-induced release of tumour necrosis factor alpha from isolated rat Kupffer cells

The higher end of the spectrum comes into play for the study of chronic inflammation calls over longer periods

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